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Vicerrectorado de Responsabilidad Social, Extensión Cultural y Servicios

Universidad de Cádiz
Edificio Constitución 1812 (Antiguo Cuartel de La Bomba)
Paseo Carlos III, 3
11003 - Cádiz
Tfno.: 956015800 Fax: 956015891



Course XI. Physics, chemistry and biology of bioluminescence



DATOS SOBRE LA ACTIVIDAD

OBJETIVOS:

The aim of this course is focused on exploring basic and applied aspects of
bioluminescence.

Priority objectives. After successfully completing the course participants:

- shall be able to understand basic mechanisms of bioluminescence of various
luminous organisms;

- shall have a good knowledge of the main principles and methods of bioluminescent
analysis and their application in biology, ecology, medicine etc.

PROGRAMA:

PROGRAMME

Preliminary Programme

Monday 26 September

8h 30 - 9h Welcome to students.

9h – 11h Lecture 1. Overview of bioluminescence: bioluminescence in nature, luciferases and luciferins, photoproteins, biochemistry and biophysics of bioluminescence, spectra in bioluminescence Speaker: Prof. Valentina Kratsyuk (SFU, Russia)

11h – 11h 30 Coffee Break.

11h 30 – 13h 30 Lecture 1.1 Determination of the main kinetics parameters of NAD(P)H:FMN-oxidoreductase + luciferase bioluminescent system; Speaker: Oleg Sutormin (SFU, Russia)

Tuesday 27 September

9h – 11h Lecture 2. Description (Enzyme and substrate/co-substrate nature, Enzyme commission number) of different luciferases (Firefly/bacterial/Cypridina/Fungi/ Renilla/ Gonyaulax/ Gaussia/ Metridia/ Vargula/ Cypridinaluciferase), and respective luciferins (structure). Photoproteins. Speaker: Prof. Valentina Kratsyuk (SFU, Russia)

11h – 11h 30 Coffee Break.

11h 30 – 13h Lecture 2.1 Determination of the kinetics features of enzyme reactions (Km, Vmax) using NAD(P)H:FMN-oxidoreductase + luciferase bioluminescent system. Speaker: Oleg Sutormin (SFU, Russia)

Wednesday 28 September

9h – 11h Lecture 3. Cloning of different bacterial lux gene cassette and their various applications in healthcare, basic research and bio-diagnostics. Speaker: Prof. Valentina Kratsyuk (SFU, Russia)

11h – 11h 30 Coffee Break.

11h 30 – 13h Lecture 3.1 Determination of pH and temperature optimums of NAD(P)H:FMN-oxidoreductase + luciferase bioluminescent system. Speaker: Oleg Sutormin (SFU, Russia)

Thursday 29 September

9h – 11h Lecture 4. Terrestrial bioluminescence: firefly and insects, bacteria, fungi, mollusks, arthropods and earthworms. Speaker: Prof. Valentina Kratsyuk (SFU, Russia)

11h – 11h 30 Coffee Break.

11h 30 – 13h 30 Lecture 4.1 Analysis of activity of NADH-dependent dehydrogenases (lactate dehydrogenase, glucose-6-phosphate dehydrogenase, alcohol dehydrogenase). Speaker: Oleg Sutormin (SFU, Russia)

Friday 30 September

9h – 11h Lecture 5. Bioluminescent analysis. Speaker: Prof. Valentina Kratsyuk (SFU, Russia)

11h – 11h 30 Coffee Break.

11h 30 – 13h 30 Lecture 5.1 Determination of marine water contamination. Speaker: Oleg Sutormin (SFU, Russia)

 

PLACE

Room nº 27 CASEM (building part B, 1 floor)


REGISTRATION

Registration:

Students interested in taking part in the course must register via the website.

Official language: English

CONTACT

Website: http://internationalsummerschool.uca.es/

Email address: iss.uca-ceimar@uca.es

Tel: +34 956015883

Address:

Universidad de Cádiz

Oficina de Relaciones Internacionales

Edificio Constitución 1812

Paseo Carlos III nº 3.

11003 Cádiz (Spain)

Lugar de celebración: Cádiz

Duración: 20 Horas

Fecha de Inicio de la Actividad: 26/09/2016

Fecha de finalización de la Actividad: 30/09/2016



DATOS ADMINISTRATIVOS

Programa al que pertenece: International III UCA International Summer School

Fecha de Inicio del Plazo de Inscripción para la Actividad: 06/07/2016

Fecha de finalización del Plazo de Inscripción para la Actividad: 30/09/2016

Precio de la actividad:
- Course XI fee excluding accommodation: 0 € (Necesita acreditar:)
- Course XI fee including accommodation: 0 € (Necesita acreditar:)

Observaciones sobre las formas de pago:

Dear Sir/Madam,

We confirm your registration in the III UCA International Summer School (Course XI). We kindly inform you that you have 3 working day time to do the payment of the fees.

Observations regarding the payment ways:

Students interested in taking part in the course must register via the website. There is NOT costs of registration.

 

Please, do not reply this e-mail.

Nº de Coordinadores: 2

Nombre del Coordinador: Valentina Kratasyuk

Universidad: Biophysical Department, School of Fundamental Biology and Biotechnology, Siberia

Nombre del segundo coordinador: Oleg Sutormin

Universidad: Laboratory of Bioluminescence, biotechnology Siberian Federal University